Effect of Addition of Different Concentrations of
Alpha Lipoic Acid to Tris Egg Yolk Citrate Glycerol Extender on
Cryopreservation of Sahiwal Bull Spermatozoa
Syed Bilal Ahmad1, Ijaz Ahmad1*,
Nazir Ahmad1, Zafar Iqbal Qureshi1, Huma Jamil1,
Qaisar Ali2 and Khurram Ashfaq3
1Department
of Theriogenology, University of Agriculture, Faisalabad; 2Semen
Production Unit, Harichand, District Charsadda; 3Department
of Clinical Medicine & Surgery, University of Agriculture,
Faisalabad, Pakistan *Corresponding author: drijazahmad_uaf@yahoo.com
Abstract
Aim of the present study was to monitor
pre-freeze and post-thaw semen quality of Sahiwal bulls after addition of
different concentrations of alpha lipoic acid (ALA) to the extender. Semen of
four Sahiwal bulls was collected two times per week for four weeks with an
artificial vagina. Ejaculates collected on each collection day from four bulls
were pooled, split into four equal parts and diluted with tris-egg
yolk-citrate-glycerol extender containing 0.500, 0.250, 0.125 and 0.000 mM
(control) ALA. Diluted samples were filled in 0.5 ml straws, frozen and stored
for at least 24 hours in liquid nitrogen. Pre-freeze and post-thaw quality was
assessed in terms of sperm motility, plasma membrane integrity, live sperm
percentage and acrosome integrity. Results indicated higher (P<0.05)
mean values for pre-freeze and post-thaw sperm motility, plasma membrane
integrity, live sperm percentage and acrosome integrity for samples diluted in
extender having 0.250 and 0.125 mM ALA compared to samples diluted in extender
containing 0.500 mM ALA and control extender. Moreover, higher mean values
(P<0.05) of these characteristics were observed in samples diluted in extender
containing 0.250 mM ALA compared to those diluted in extender containing 0.125
mM ALA. However, samples diluted with 0.500 mM ALA showed lower (P<0.05)
pre-freeze and post-thaw semen quality than control samples. It was concluded
that ALA at a concentration of 0.250 mM significantly improved pre-freeze
and post-thaw characteristics of Sahiwal bull semen. However, field trials
regarding effects of adding different concentrations of ALA to semen extender on
fertility of frozen-thawed semen are necessary before making any recommendations
in this regard.
To Cite This Article: Ahmad SB, Ahmad I, Ahmad N, Qureshi ZI, Jamil H,
Ali Qand Ashfaq K, 2018. Effect of addition of different
concentrations of alpha lipoic acid to tris egg yolk citrate glycerol extender
on cryopreservation of Sahiwal bull spermatozoa. Pak Vet J, 38(3): 301-305.
http://dx.doi.org/10.29261/pakvetj/2018.060