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Antidiabetic Activity of Date Seed Methanolic Extracts in Alloxan-Induced Diabetic Rats

Seyed Abdulmajid Ayatollahi1,2, Mehdi Sharifi-Rad3, Amir Roointan4, Navid Baghalpour1*, Bahare Salehi5*Zabta Khan Shinwari6,7 *, Ali Talha Khalil8 and Javad Sharifi-Rad1*

1Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 3Zabol Medicinal Plants Research Center, Zabol University of Medical Sciences, Zabol, Iran; 4Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; 5Student Research Committee, School of Medicine, Bam University of Medical Sciences, Bam, Iran; 6Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan; 7Qarshi University, Lahore, Pakistan; 8Department of Eastern Medicine and Surgery Qarshi University Lahore Pakistan
*Corresponding author:;;;


Phoenix dactylifera. L. )date palm ( is one of the main fruits in North of Africa and Middle East. The date seeds are considered as wastes despite of their medicinal properties. Different seed preparations are used as expectorant, anti-diarrheic, hypoglycemic, tonic, and aphrodisiac agents. We established the anti-diabetic and anti- lipidemic activities of methanolic extracts of date seed in alloxan-induced diabetic rats. Animals were divided in to 6 groups (each group contained six animals), comprising of control group and diabetic rats. Animals were treated with different amounts of extract. To assess the hypolipidemic, and anti-hyperglycemic activity, cholesterol serum levels, low density lipoprotein (LDL), high density lipoprotein (HDL), creatinine, urea and alkaline phosphatase were measured in serum of the treated animals. The anti-diabetic potential was investigated through the levels of glucose and body weight. Moreover, to assess the extract’s safety, acute toxicity test was performed. In comparison with control group, significant reductions were observed in LDL, cholesterol, as well as blood glucose levels in diabetic rats that received date seed extract. Extract supplemented diabetic rats also showed a better tolerance to glucose, as well as reduced amounts of creatinine, urea, and alkaline phosphatase in their serum samples. In addition, no toxicity (acute toxicity), was detected even after high dosage of extract administration. Our results confirmed the anti-lipidemic and anti-diabetic potentials of date seed methanolic extract in diabetic rats.

To Cite This Article: Ayatollahi SA, Sharifi-Rad M, Roointan A, Baghalpour N, Salehi B, Shinwari ZK, Khalil AT and Sharifi-Rad J, 2019. Antidiabetic activity of date seed methanolic extracts in alloxan-induced diabetic rats. Pak Vet J, 39(4): 583-587.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)