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Construction of a Triple Gene-Deleted Live Vaccine Candidate against Pseudorabies Virus Using CRISPR/Cas9 and Cre/LoxP System-Based Strategy
Muping Wang, Longtao Wang* and Quan Zhao*

College of Animal Sciences and Technology, Jilin Agricultural University, 2888 Xincheng Street, Changchun 130118, China
*Corresponding author:;


The immune protective effects of pseudorabies virus (PRV) vaccines in the market have gradually reduced and they have failed to provide complete protection against the new PRV variant. In this study, a triple gene-deleted live PRV strain–rZDΔTK-gE-gI was successfully constructed by simultaneously knocking out the three major virulence genes (gE/gI and TK) with CRISPR/Cas9 and Cre/LoxP gene editing system and low melting point agarose purification method. After challenge with the virulent PRV variant, all the 3-week-old piglets vaccinated with the rZDΔTK-gE-gI PRV vaccine candidate survived without any clinical symptoms, whereas all the unvaccinated piglets exhibited PRV respiratory and neurological signs with 100% mortality rate within 7 days post infection. High levels of anti-gB antibodies were induced in the vaccinated piglets after vaccination with the rZDΔTK-gE-gI vaccine candidate, which elicited a better immune protective effect than the classical strain Bartha-K61. Therefore, the triple gene-deleted live PRV vaccine candidate is expected to control the current outbreak of pseudorabies caused by the PRV variants.

To Cite This Article: Wang M, Wang L and Zhao Q, 2021. Construction of a triple gene-deleted live vaccine candidate against pseudorabies virus using CRISPR/Cas9 and Cre/LoxP system-based strategy. Pak Vet J, 41(4): 493-498.


ISSN 0253-8318 (Print)
ISSN 2074-7764 (Online)