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First Report of Antimicrobial Resistance of Mannheimia haemolytica from Phan Rang Sheep in Vietnam
Phu Van Nguyen1, Cong Tuan Le2, Xuan Tuy Thi Ho1, Phuc Hung Truong3, Bui Van Loi4 and Kim Cuc Thi Nguyen1*

1Institute of Biotechnology, Hue University, Road 10, Phu Thuong, Hue city 530000, Vietnam
2Department of Environmental Science, University of Sciences, Hue University, Hue city 530000, Vietnam
3Faculty of Biotechnology, Thai Nguyen University of Sciences, Tan Thinh Ward, Thai Nguyen City 24000, Vietnam
4Hue University, 03 Le Loi St., Hue City, 530000, Vietnam
*Corresponding author:


Mannheimia haemolytica is the principal agent associated with respiratory diseases in different animals, however, the prevalence and antimicrobial resistance of this Gram-negative bacteria in Phan Rang sheep, a local sheep of Vietnam, are still unknown. In the present study, 31 M. haemolytica isolates were obtained from 170 samples from clinically sick and healthy sheep. A higher prevalence of M. haemolytica was detected in clinically sick samples (25/85) than in healthy ones (6/85). The antimicrobial susceptibility of 31 M. haemolytica isolates was determined using eleven antibiotics. Among them, M. haemolytica strains were susceptible to ofloxacin (100%), ciprofloxacin (96.77%), enrofloxacin (93.55%), and chloramphenicol (90.32%), whereas, they were resistant to oxytetracycline (74.19%), tulathromycin (70.97%), erythromycin (67.74%), penicillin (51.61%), and ampicillin (41.93%). More than 74% of M. haemolytica isolates exhibited multidrug resistance, of which 54.84% of isolates resisted 3 to 4 antibiotics, and 19.35% of isolates resisted 5-6 antibiotics. A good correlation between genotype and resistance phenotype for oxytetracycline was detected as 25/31 isolates carrying at least one gene (tetB or/and tetH). On the other hand, a poor correlation between genotype and phenotype for β-lactam, and macrolide antibiotic groups were observed as none of the resistance phenotype harboring blaTEM, erm42, ermB, and mphE gene. These findings provide the first reported evidence of the prevalence and antibiotic resistance of M. haemolytica that have contributed to Phan Rang sheep respiratory disease in central Vietnam.

To Cite This Article: Nguyen PV, Le CT, Ho XTT, Truong PH, Loi BV and Nguyen KCT, 2023. First report of antimicrobial resistance of Mannheimia haemolytica from phan rang sheep in vietnam. Pak Vet J, 43(1): 41-48.


ISSN 0253-8318 (Print)
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